Maybe you are just missing a small step. When you granted the account read access, did you check that it replaced settings in all subdirectories. Also it may require an IIS reset after changing the access rights.
Just as an FYI. After rebuilding servers and spending endless hours troubleshooting a 401.3 error after changing the AppPool identity to a domain account, we came to find out that a GPO was killing IIS. Make sure you test with a server in the "Computer" container and that no GPO's are changing restricted groups outside of Administrators.
In our case, NT Authority\Authenticated Users was removed from the local USERS group and it broke IIS complete.
I would check what is the exact status code of the 401 error IIS 7 Status Code Once you know the exact error code, it will be easy for to troubleshoot the exact issue is.
I would also run Process Monitor and see if there are any "ACCESS DENIED" using the Filter.
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也许你只是错过了一小步。当您授予帐户读取权限时，您是否检查它是否替换了所有子目录中的设置。此外，更改访问权限后可能需要重置 IIS。
Maybe you are just missing a small step. When you granted the account read access, did you check that it replaced settings in all subdirectories. Also it may require an IIS reset after changing the access rights.
2008 R2 IIS 7.5
仅供参考。在重建服务器并花费无数时间对 AppPool 身份更改为域帐户后的 401.3 错误进行故障排除后，我们发现 GPO 正在杀死 IIS。确保您使用“计算机”容器中的服务器进行测试，并且没有 GPO 更改管理员之外的受限组。
在我们的例子中，NT Authority\Authenticated Users 已从本地 USERS 组中删除，这导致 IIS 完全崩溃。
2008 R2 IIS 7.5
Just as an FYI. After rebuilding servers and spending endless hours troubleshooting a 401.3 error after changing the AppPool identity to a domain account, we came to find out that a GPO was killing IIS. Make sure you test with a server in the "Computer" container and that no GPO's are changing restricted groups outside of Administrators.
In our case, NT Authority\Authenticated Users was removed from the local USERS group and it broke IIS complete.
我会检查 401 错误的确切状态代码是什么 IIS 7 状态代码
一旦知道确切的错误代码，就可以轻松地解决确切的问题。
我还会运行 进程监视器 并查看是否有任何使用过滤器的“访问被拒绝”。
问候，
维韦克。
I would check what is the exact status code of the 401 error IIS 7 Status Code
Once you know the exact error code, it will be easy for to troubleshoot the exact issue is.
I would also run Process Monitor and see if there are any "ACCESS DENIED" using the Filter.
Regards,
Vivek.